Thermodynamics of alpha-Chymotrypsin-Inhibitor Complex Formation

Thermodynamics of alpha- and beta-structure formation in proteins.

An atomic protein model with a minimalistic potential is developed and then tested on an alpha-helix and a beta-hairpin, using exactly the same parameters for both peptides. We find that melting curves for these sequences to a good approximation can be described by a simple two-state model, with parameters that are in reasonable quantitative agreement with experimental data. Despite the apparen...

Complex formation of guanidinated bovine trypsin inhibitor (Kunitz) with trypsin, chymotrypsin and trypsinogen as studied by the spin-label technique.

2.1. Materials One approach to circumvent this difficulty is to introduce an indirect signal by coupling an indicator reaction to the reaction of interest. Examples are monitoring the enzymatic activity of free enzyme [2] or following the displacement of proflavin from the enzyme when the inhibitor is bound [3]. With all these indirect measurements careful studies as to possible side reactions ...

Thermodynamics and Kinetics of Spiro-Heterocycle Formation Mechanism: Computational Study

Reaction mechanism among indoline-2,3-dione, pyrrolidine-2-carboxylic acid and (Z)-2-(1-(2-hydroxynaphthalen-1-yl)ethylidene)hydroxycarboxamide to form 1’-((((aminooxy)carbonyl)amino)methyl)-2’-(1-hydroxynaphthalen-2-yl)-2’-methyl-1’,2’,5’,6’,7’,7a’-hexahydrospiro[indoline-3,3’-pyrrolo[1,2-a]imidazole-2-one was investigated using density functional theory (DFT) at B3LYP basis theory. The three-...

Effectiveness of Alpha Chymotrypsin on the Treatment of Severe Paralysis due to Bungarus Multicinctus Snakebite

[Intracapsular cataract extraction using alpha-chymotrypsin].

ALPHA-CHYMO-TRYPSIN (A.C.T.) is an enzyme with fibrinolytic and proteolytic properties which is prepared from bovine pancreas. It was discovered by Dr. Joaquin Barraquer in 1957 that the substance had a selective solvent action on the zonule supporting the lens. After extensive animal and postmortem experiments he applied the discovery to the living human eye with important results. He found th...